Fluorescence Studies of Nucleotides and Polynucleotides
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چکیده
منابع مشابه
Fluorescence Studies of Nucleotides and Polynucleotides
Deazanebularin, a cytotoxic nucleoside, is fluorescent at ambient temperature and at neutral pH. Some of its fluorescence properties and those of its phosphorylated derivatives are reported here. Deazanebularin nucleotides are substrates for Escherichia coli polynucleotide phosphorylase and RNA polymerase. With RNA polymerase deazanebularin triphosphate acts as an ambiguous substrate in that it...
متن کاملpostnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)
1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...
Excimer fluorescence of dinucleotides, polynucleotides, and DNA.
The ultraviolet-absorption spectrum of DNA closely resembles that of a mixture of its constituent nucleotidesl apart from the well-known hypochromism.2' I On the other hand, the emission spectra of DNA and its constituent nucleotides are qualitatively different. The phosphorescence from the excited triplet state of DNA has been shown4' to originate from thymine residues which after excitation h...
متن کاملFluorescence studies of the interaction of pyrenylmethyl tributylphosphonium bromide with double-strand polynucleotides.
The interaction between pyren-1-ylmethyl tri-n-butylphosphonium bromide (PMTP), a water-soluble cationic pyrene derivative and the double-strand polynucleotides Poly[dA-dT], Poly[dA].Poly[dT], Poly[dG-dC] and Poly[dG].Poly[dC] was studied using UV-Vis absorption and fluorescence spectroscopy. The PMTP probe interacts with polynucleotides through both weak and intercalative binding, evidenced th...
متن کاملBinding of nucleotides by T4 DNA ligase and T4 RNA ligase: optical absorbance and fluorescence studies.
The interaction of nucleotides with T4 DNA and RNA ligases has been characterized using ultraviolet visible (UV-VIS) absorbance and fluorescence spectroscopy. Both enzymes bind nucleotides with the K(d) between 0.1 and 20 microM. Nucleotide binding results in a decrease of absorbance at 260 nm due to pi-stacking with an aromatic residue, possibly phenylalanine, and causes red-shifting of the ab...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91833-8